WIBS-5
Wideband Integrated Bioaerosol Sensor
The WIBS-5 is the world’s only instrument for real-time, single particle measurement of atmospheric bacteria, molds, pollen, and other bioaerosols. WIBS-5 measures fluorescence to infer the presence of biological material in particles and provides detailed data on size, relative measure of shape, and fluorescent properties to enable classification of pollen, bacteria, and fungi.
TALK TO AN EXPERTMeasure trace amounts of mold and other bioaerosols in the atmosphere.
Get an instrument optimized for detection of bioaerosols, tryptophan, and NADH using two excitation wavelengths and two emission bands.
Get specific data for particles from 0.5 to 30 µm, as well as particle time-of-flight to detect coincidence; configurable to measure larger particles such as pollens and fungal spores.
Enables selection of filter criteria for both full and partial measurements. Filter data using a wide variety of criteria: particle size, asymmetry, and concentration; fluorescent excitation and emission; or ratios of any of these parameters.
Measure particles in real time and see housekeeping parameters to monitor instrument health and enhance operational uptime.
Get more flexibility for your different research applications. Collect measurements and data on the ground or in the air.
WIBS-5 Software
Two software packages are available for the WIBS-5: the standard software system and the WIBS Data Analysis Toolkit.
Standard Software
The WIBS-5 graphical user interface features several components. The main window shown below details measured particles in real time and gives information on several housekeeping parameters.
WIBS-5 Software
WIBS Data Analysis Toolkit
The WIBS toolkit allows for loading, processing, and inspection of data generated by the WIBS-5/NEO. The toolkit’s functionality includes the following:
- Loading single and multiple raw data files containing particle-by-particle fluorescence, size, asymmetry factor, and other data
- Converting particle-by-particle data into time-resolved particle concentrations and size distributions
- Basic plotting capability
The toolkit window is shown here.
Particle light-scattering and fluorescence detection
- Particles are excited by 280 nm and 370 nm flashlamps.
- Forward-scattered light from a 635 nm diode laser determines particle size and asphericity.
- Two emission bands give a detailed excitation-emission matrix which is highly sensitive to common fluorophores such as tryptophan and NADH.
| Parameter | Specification |
|---|---|
| Maximum Concentration | 466 particles/cm3 for fluorescent particle counting (10% coincidence) 9,500 particles/cm3 for sizing and counting (10% coincidence) |
| Fluorescence Excitation | 280 nm, 370 nm |
| Fluorescence Emission | 310-400 nm, 420-650 nm |
| Flow Rate | Sample: 0.3 L/min |
| Particle Size Range | 0.5 to 30 µm |
| Sheath | 2.1 L/min |
| Power Reqiurements | 100W, 90-230 VAC or 24 VDC at 3.0A |
| Weight | 27.5 lbs |
| Dimensions | 17.75"W x 14.25"L x 9.5"H (add 2" for inlet at top) |
The Lifecycle Care Program ensures WIBS-5 integrity and performance vital to your research mission for 3 years or 3 calls (whichever comes first). When conducting complex and time intensive laboratory or field measurements it is critical to avoid down time, and ensure data collection accuracy. The program consists of 3 key deliverables for each year or call:
- Instrument Logistics
- Instrument Integrity
- Instrument Performance
Lifecycle Care Program Includes:
LOGISTICS
- Shipping to and from Droplet Measurement Technologies facility
- Customs handling, including CARNET ATA if required
INTEGRITY
- Incoming instrument evaluation and health check
PERFORMANCE
- Software and firmware validation
- 100%-unit functionality test
- Calibration data
- Calibration report
- Certificate of calibration
At times, our technician might discover an instrument issue, which might not be covered by the Lifecycle Care Program or by our product warranty. In such case we will reach out to you with a service & repair estimate to allow you to provide us with the next steps of action.
Cluster analysis of WIBS single-particle bioaerosol data
Hierarchical agglomerative cluster analysis was performed on single-particle multi-spatial data sets comprising optical diameter, asymmetry and three different fluorescence measurements, gathered using two dual Wideband Integrated Bioaerosol Sensors (WIBSs).
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On-line measurement of fluorescent aerosols near an industrial zone in the Yangtze River Delta region using a wideband integrated bioaerosol spectrometer
In this work, we present on-line fluorescent aerosol measurements by the wideband integrated bioaerosol spectrometer (WIBS-4A) near an industrial zone in Nanjing, a megacity in the Yangtze-River-Delta (YRD) region.
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Fluorescent biological aerosol particles measured with the Waveband Integrated Bioaerosol Sensor WIBS-4: laboratory tests combined with a one year field study
This study aims to investigate the sensitivity of WIBS-4 to biological and non-biological aerosols and detection of biological particles in the ambient aerosol.
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